| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williams, Erik D (14)
|
MAUN |
51.52 |
52.77 |
1.25 |
| 2 |
Brennan, Ryan K (14)
|
MAGPAC |
53.56 |
53.54 |
-0.02 |
| 3 |
Sheppard, Dominick D (14)
|
MAGPAC |
53.81 |
54.93 |
1.12 |
| 4 |
Holden, Marquis I (14)
|
MASERP |
54.32 |
54.99 |
0.67 |
| 5 |
Bokarev, Andrew (14)
|
MANRG |
56.03 |
55.09 |
-0.94 |
| 6 |
Maier, Andrew D (13)
|
MAGPAC |
55.52 |
55.44 |
-0.08 |
| 7 |
Wells, Jesse (14)
|
MAPENN |
55.26 |
55.70 |
0.44 |
| 8 |
Cedeno, Luis Enrique B (13)
|
MAGPAC |
59.23 |
55.97 |
-3.26 |
| 9 |
Brown, Sultan M (14)
|
MASERP |
1:00.44 |
57.24 |
-3.20 |
| 10 |
Nguyen, Dustin H (13)
|
MAPVA |
1:01.80 |
58.07 |
-3.73 |
| 11 |
Pasco, Andrew H (13)
|
MAYBCC |
1:00.79 |
59.26 |
-1.53 |
| 12 |
Gibson, Sa'ood T (14)
|
MASERP |
1:01.39 |
59.89 |
-1.50 |
| 13 |
Beaver, Austin W (13)
|
MAJRAY |
1:04.40 |
1:00.57 |
-3.83 |
| 14 |
Keagy, Ryan P (14)
|
MAUN |
1:08.63 |
1:09.33 |
0.70 |